Cleaning and disinfection of surfaces is another important infection control procedure that should be practised by all dentists to prevent the spread of microorganisms. The transmission of dental healthcare associated infections occur when the gloved or ungloved hands of a dental care provider comes in contact with a contaminated surface or equipment. 

The transmission of such infection can also occur through patient contact with contaminated surfaces or equipment. Proper cleaning and disinfection of all clinical contact surfaces, e.g. instrument tray, dental chair, dental light and its handle, switches, door knobs or handles, drawer handles and countertops; and other surfaces, should be done after each patient and before the next patient enters the examination or surgery room. 

Protective plastic sheets, covers or bags are sometimes used to protect such surfaces and equipment. When such protection of surfaces and equipment are used, they should be removed, discarded and replaced after each patient. Whether a practice uses protective barriers or surface disinfection is a personal choice, but many experts advocate the use of surface barriers, especially for surfaces that are difficult to clean (e.g. light handles and control panels).

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